Patients will be screened for infection such as HIV, hepatitis, or syphilis. The male patient will provide a semen sample through masturbation.
If the patient does not produce sperm in the ejaculation, a sperm sample may be taken from his testes. The collected sperm samples are separated into multiple containers. Skilled lab technicians will use cryoprotectant, a substance used to protect the sperm from freezing damage. The frozen vials of sperm are stored in the lab until the patient is ready to use them again.